Growth Hormone Cloning

This program maps the molecular techniques required to isolate, clone and express a foreign gene in a prokaryote model.
The session involves students cloning and expressing the Human Growth Hormone gene in bacteria. Concepts covered in this program include:
• PCR
• Restriction enzyme digest
• DNA ligase action
• Plasmids as vectors
• Bacterial promotors
• Ethical considerations of gene cloning
Students will be hands on through the majority of the session and will be guided in small groups by an experienced mentor.

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