Sprinters Gene

This program looks at how to screen for the ACTN3 Gene (or Sprinter’s Gene) before exploring the concept of gene cloning.

In the first session, students develop a technique to screen for the Sprinter’s Allele of the gene. Concepts covered in this session include:

• Restriction enzyme digests
• Gel electrophoresis (both protein and DNA)
• DNA sequencing

The second session involves students cloning and expressing the Sprinter’s Allele in a bacteria model. Concepts covered in this session include:

• PCR
• Restriction enzyme digests
• Plasmid vectors
• Bacterial promotors

Students will be hands on through the session and be guided by a mentor in small groups.

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